Name: Biotinylated Human Nectin-4 Protein (NEC-HM404B)
Brand: KactusBio
SKU: NEC-HM404B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human Nectin-4 Protein (NEC-HM404B)

Immobilized Biotinylated Human Nectin-4, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA.

Biotinylated Human Nectin-4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

The purity of Bioyintlated Human Nectin-4 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human Nectin-4, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gly32-Val351

Catalog #NEC-HM404B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human Nectin-4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly32-Val351.
Product Background	Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Protein	Nectin-4
Synonyms	EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4
Accession	Q96NY8
Species	Human
Biotinylated	yes
Amino Acid Range	Gly32-Val351
Molecular Weight	The protein has a predicted MW of 37.2 kDa. Due to glycosylation, the protein migrates to 46-48 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human Nectin-4, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human Nectin-4, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA.
	Biotinylated Human Nectin-4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	The purity of Bioyintlated Human Nectin-4 is greater than 95% as determined by SEC-HPLC.