Biotinylated Human Nectin-4 Protein (NEC-HM404B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human Nectin-4, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Nectin4 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly32-Val351

Catalog: NEC-HM404B

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Product Description Recombinant Biotinylated Human Nectin-4 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gly32-Val351.
Product Category Recombinant Protein
Target Nectin-4
Synonyms EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4
Gene/Protein ID Q96NY8
Species Human
Exact Sequence Gly32-Val351
Molecular Weight The protein has a predicted MW of 37.2 kDa. Due to glycosylation, the protein migrates to 46-48 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human Nectin-4, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Nectin4 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human Nectin-4, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Nectin4 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA.
The purity of Bioyintlated Human Nectin-4 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Nectin-4 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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