Biotinylated Human Nectin-4 Protein (NEC-HM404B)
Biotinylated Human Nectin-4 Protein (NEC-HM404B)
Biotinylated Human Nectin-4 Protein (NEC-HM404B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human Nectin-4, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly32-Val351
Catalog #NEC-HM404B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human Nectin-4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly32-Val351.
Product Background
Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.
Product Category
Recombinant Protein
Target
Nectin-4
Synonyms
EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Gly32-Val351
Molecular Weight
The protein has a predicted MW of 37.2 kDa. Due to glycosylation, the protein migrates to 46-48 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human Nectin-4, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human Nectin-4, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA.
Biotinylated Human Nectin-4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Bioyintlated Human Nectin-4 is greater than 95% as determined by SEC-HPLC.

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