Biotinylated Human PD-1/PDCD1 Protein (PD1-HM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human PD-1, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 23.5ng/ml determined by ELISA (QC Test). The affinity constant of 2.356 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu25-Gln167
Catalog #PD1-HM401B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human PD-1/PDCD1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu25-Gln167.
Product Background
Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.
Product Category
Recombinant Protein
Target
PD-1/PDCD1
Synonyms
PDCD1; PD1; CD279; SLEB2; PD-1
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Leu25-Gln167
Molecular Weight
The protein has a predicted MW of 18.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human PD-1, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 23.5ng/ml determined by ELISA (QC Test). The affinity constant of 2.356 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-1, His-Avi Tag with an affinity constant of 2.356 uM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human PD-1, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 23.5ng/ml determined by ELISA (QC Test).
Biotinylated Human PD-1 on Bis-Tris PAGE under reduced. The purity is greater than 95%.
The purity of Biotinylated Human PD-1 is greater than 95% as determined by SEC-HPLC.
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