Biotinylated Human PD-1/PDCD1 Protein (PD1-HM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human PD-1, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 23.5ng/ml determined by ELISA (QC Test). The affinity constant of 2.356 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu25-Gln167
Catalog #PD1-HM401B
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Product Description
 Recombinant Biotinylated Human PD-1/PDCD1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu25-Gln167.
Product Background
 Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.
Product Category
 Recombinant Protein
Target
 PD-1/PDCD1
Synonyms
 PDCD1; PD1; CD279; SLEB2; PD-1
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Leu25-Gln167
Molecular Weight
 The protein has a predicted MW of 18.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human PD-1, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 23.5ng/ml determined by ELISA (QC Test). The affinity constant of 2.356 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-1, His-Avi Tag with an affinity constant of 2.356 uM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human PD-1, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 23.5ng/ml determined by ELISA (QC Test).
Biotinylated Human PD-1 on Bis-Tris PAGE under reduced. The purity is greater than 95%.
The purity of Biotinylated Human PD-1 is greater than 95% as determined by SEC-HPLC.

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