Biotinylated Human PD-1/PDCD1 Protein (PD1-HM401B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human PD-1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PD-1 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA. The affinity constant of 2.356 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Leu25-Gln167

Catalog: PD1-HM401B

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Product Description Recombinant Biotinylated Human PD-1/PDCD1 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Leu25-Gln167.
Product Category Recombinant Protein
Target PD-1/PDCD1
Synonyms PDCD1; PD1; CD279; SLEB2; PD-1
Gene/Protein ID Q15116-1
Species Human
Exact Sequence Leu25-Gln167
Molecular Weight The protein has a predicted MW of 18.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human PD-1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PD-1 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA. The affinity constant of 2.356 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-1, His-Avi Tag with an affinity constant of 2.356 μM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human PD-1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PD-1 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA.
The purity of Biotinylated Human PD-1 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human PD-1 on Tris-Bis PAGE under reduced . The purity is greater than 95%.

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