Product Description |
Recombinant Biotinylated Human Syndecan-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln23-Gly254. |
Product Background |
Liver diseases such as liver cirrhosis, primary and metastatic liver cancers are still a major medical challenge. Syndecan-1 is one of the most important proteoglycans in the liver. Syndecan-1 is normally expressed on the surfaces of hepatocytes and cholangiocytes. Due to liver diseases the amount of syndecan-1 increases in the liver. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
Syndecan-1 |
Synonyms |
Syndecan-1; SYND1; CD138; SDC1; SDC; SDC1 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gln23-Gly254 |
Molecular Weight |
The protein has a predicted MW of 26.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized BBiotinylated Human Syndecan-1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Syndecan-1 Antibody, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized BBiotinylated Human Syndecan-1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Syndecan-1 Antibody, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test). |
|
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Biotinylated Human Syndecan-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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