Biotinylated Human Syndecan-1 Protein (SYN-HM401B)
|Recombinant Biotinylated Human Syndecan-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln23-Gly254.
|Liver diseases such as liver cirrhosis, primary and metastatic liver cancers are still a major medical challenge. Syndecan-1 is one of the most important proteoglycans in the liver. Syndecan-1 is normally expressed on the surfaces of hepatocytes and cholangiocytes. Due to liver diseases the amount of syndecan-1 increases in the liver.
|Syndecan-1; SYND1; CD138; SDC1; SDC; SDC1
|The protein has a predicted MW of 26.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
|Immobilized Anti-Syndecan-1 Antibody at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Syndecan-1, His Tag with the EC50 of 0.12ug/ml determined by ELISA. See testing image for detail.
|> 95% as determined by Tris-Bis PAGE
|Less than 1EU per ug by the LAL method.
|Shipped at ambient temperature.
|Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
|Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
|Stability And Storage
|Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.