Biotinylated Human Syndecan-1 Protein (SYN-HM401B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized BBiotinylated Human Syndecan-1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Syndecan-1 Antibody, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gln23-Gly254

Catalog: SYN-HM401B

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Product Description Recombinant Biotinylated Human Syndecan-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln23-Gly254.

Product Background

 Liver diseases such as liver cirrhosis, primary and metastatic liver cancers are still a major medical challenge. Syndecan-1 is one of the most important proteoglycans in the liver. Syndecan-1 is normally expressed on the surfaces of hepatocytes and cholangiocytes. Due to liver diseases the amount of syndecan-1 increases in the liver.

Product Category

 Recombinant Protein

Target

 Syndecan-1
Synonyms Syndecan-1; SYND1; CD138; SDC1; SDC; SDC1
Accession

Species

 Human

Amino Acid Range

 Gln23-Gly254
Molecular Weight The protein has a predicted MW of 26.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized BBiotinylated Human Syndecan-1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Syndecan-1 Antibody, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

 C-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized BBiotinylated Human Syndecan-1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Syndecan-1 Antibody, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test).
Biotinylated Human Syndecan-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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