Product Description |
Recombinant Biotinylated Human TPBG/5T4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser32-Ser355. |
Product Background |
The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
TPBG/5T4 |
Synonyms |
M6P1; 5T4AG; WAIF1; TPBG; 5T4 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ser32-Ser355 |
Molecular Weight |
The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human TPBG, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Immobilized Biotinylated Human TPBG, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test). |
|
|
Biotinylated Human TPBG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human TPBG is greater than 95% as determined by SEC-HPLC. |
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