Biotinylated Human TPBG/5T4 Protein (5T4-HM401B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-TPBG Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TPBG, His Tag with the EC50 of 84.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser32-Ser355

Catalog: 5T4-HM401B

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Product Description Recombinant Biotinylated Human TPBG/5T4 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Ser32-Ser355.
Product Category Recombinant Protein
Target TPBG/5T4
Synonyms M6P1; 5T4AG; WAIF1; TPBG; 5T4
Gene/Protein ID Q13641-1
Species Human
Exact Sequence Ser32-Ser355
Molecular Weight The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-TPBG Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TPBG, His Tag with the EC50 of 84.3ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-TPBG Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TPBG, His Tag with the EC50 of 84.3ng/ml determined by ELISA.
The purity of Biotinylated Human TPBG is greater than 95% as determined by SEC-HPLC.
Biotinylated Human TPBG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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