Biotinylated Human TRAIL R2/DR5/TNFRSF10B Protein (TRL-HM4R2B)
Biotinylated Human TRAIL R2/DR5/TNFRSF10B Protein (TRL-HM4R2B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human TRAIL R2, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TRAIL R2 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ile56-Glu182
Catalog #TRL-HM4R2B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human TRAIL R2/DR5/TNFRSF10B Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ile56-Glu182.
Product Background
DR5, also called TRAIL R2, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). DR5 is a receptor for the cytotoxic ligand TNFSF10/TRAIL. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis.
Product Category
Recombinant Protein
Target
TRAIL R2/DR5/TNFRSF10B
Synonyms
CD262; DR5; DR5TRICK2B; Fas-like protein; KILLER/DR5; TNFRSF10B; TRAIL R2; TRAIL receptor 2; TRAILR2; TRICK2; TRICK2A; TRICKB; ZTNFR9; KILLER
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Ile56-Glu182
Molecular Weight
The protein has a predicted MW of 17.2 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human TRAIL R2, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TRAIL R2 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human TRAIL R2, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TRAIL R2 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA.
Biotinylated Human TRAIL R2 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Biotinylated Human TRAIL R2 is greater than 95% as determined by SEC-HPLC.

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