Biotinylated Human TRAIL R2/DR5/TNFRSF10B Protein (TRL-HM4R2B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized BBiotinylated Human TRAIL R2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Trail R2 Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ile56-Glu182

Catalog: TRL-HM4R2B

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Product Description Recombinant Biotinylated Human TRAIL R2/DR5/TNFRSF10B Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Ile56-Glu182.
Product Category Recombinant Protein
Target TRAIL R2/DR5/TNFRSF10B
Synonyms CD262; DR5; DR5TRICK2B; Fas-like protein; KILLER/DR5; TNFRSF10B; TRAIL R2; TRAIL receptor 2; TRAILR2; TRICK2; TRICK2A; TRICKB; ZTNFR9; KILLER
Gene/Protein ID O14763-1
Species Human
Exact Sequence Ile56-Glu182
Molecular Weight The protein has a predicted MW of 17.2 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result.
Activity Immobilized BBiotinylated Human TRAIL R2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Trail R2 Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized BBiotinylated Human TRAIL R2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Trail R2 Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA.
The purity of Biotinylated Human TRAIL R2 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human TRAIL R2 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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