Biotinylated Human TROP-2/TACSTD2 Protein (TRP-HM421B)
Biotinylated Human TROP-2/TACSTD2 Protein (TRP-HM421B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human TROP-2, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TROP-2 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: His27-Thr274
Catalog #TRP-HM421B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human TROP-2/TACSTD2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His27-Thr274.
Product Background
Trop-2,also known as epithelial glycoprotein-1 antigen (EGP-1),is a protein that in humans is encoded by the TACSTD2 gene.Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness.
Product Category
Recombinant Protein
Target
TROP-2/TACSTD2
Synonyms
EGP1; EGP-1; TROP2; GA733-1; gp50; T16; TACSTD2; TROP-2; M1S1; TACD2
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
His27-Thr274
Molecular Weight
The protein has a predicted MW of 30.5 kDa. Due to glycosylation, the protein migrates to 46-55 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human TROP-2, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TROP-2 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human TROP-2, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TROP-2 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA.
Biotinylated Human TROP-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human TROP-2 is greater than 95% as determined by SEC-HPLC.
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