Biotinylated Mouse BCMA/TNFRSF17 Protein (BCM-MM417B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse APRIL, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 3.4ng/ml determined by ELISA (QC Test). The affinity constant of 5.64 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Met1-Thr49
Catalog #BCM-MM417B
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Product Description
 Recombinant Biotinylated Mouse BCMA/TNFRSF17 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met1-Thr49.
Product Background
 B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene.TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF).
Product Category
 Recombinant Protein
Target
 BCMA/TNFRSF17
Synonyms
 CD269; TNFRSF17; BCMA; BCM; TNFRSF13A
Accession
Species
 Mouse
Biotinylated
 yes
Amino Acid Range
 Met1-Thr49
Molecular Weight
 The protein has a predicted MW of 8.5 kDa. Due to glycosylation, the protein migrates to 12-16 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Mouse APRIL, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 3.4ng/ml determined by ELISA (QC Test). The affinity constant of 5.64 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Mouse BCMA, His-Avi Tag with an affinity constant of 5.64 uM as determined in SPR assay (Biacore T200).
Immobilized Mouse APRIL, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 3.4ng/ml determined by ELISA (QC Test).
Biotinylated Mouse BCMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Mouse BCMA is greater than 95% as determined by SEC-HPLC.

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