Canine CD40/TNFRSF5 Protein (CD4-DM140)
Canine CD40/TNFRSF5 Protein (CD4-DM140)
Canine CD40/TNFRSF5 Protein (CD4-DM140)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Canine CD40, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD40 Ligand Trimer, hFc Tag with the EC50 of 29.4ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Glu21-Ala194
Catalog #CD4-DM140
Regular price $315.00
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Product Description
Recombinant Canine CD40/TNFRSF5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu21-Ala194.
Product Background
CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.
Product Category
Recombinant Protein
Target
CD40/TNFRSF5
Accession
Species
Canine
Biotinylated
no
Amino Acid Range
Glu21-Ala194
Molecular Weight
The protein has a predicted MW of 20.14 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Canine CD40, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD40 Ligand Trimer, hFc Tag with the EC50 of 29.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Canine CD40, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD40 Ligand Trimer, hFc Tag with the EC50 of 29.4ng/ml determined by ELISA (QC Test).
Canine CD40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Canine CD40 is greater than 95% as determined by SEC-HPLC.

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