Name: Canine CD40/TNFRSF5 Protein (CD4-DM140)
Brand: KactusBio
SKU: CD4-DM140-100UG
Price: 315.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Canine CD40, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD40 Ligand Trimer, hFc Tag with the EC50 of 29.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Glu21-Ala194

Catalog #CD4-DM140

Size

100UG

500UG

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Product Description	Recombinant Canine CD40/TNFRSF5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu21-Ala194.
Product Background	CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	CD40/TNFRSF5
Synonyms	CD40; CD40L receptor;- TNFRSF5; CD40 antigen; CD40 molecule; CDw40; MGC9013;
Accession	Q7YRL5
Species	Canine
Biotinylated	no
Amino Acid Range	Glu21-Ala194
Molecular Weight	The protein has a predicted MW of 20.14 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Canine CD40, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD40 Ligand Trimer, hFc Tag with the EC50 of 29.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Canine CD40, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD40 Ligand Trimer, hFc Tag with the EC50 of 29.4ng/ml determined by ELISA (QC Test).
	Canine CD40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Canine CD40 is greater than 95% as determined by SEC-HPLC.

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