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Canine LRRC15/LIB Protein (LRR-DM415) Summary
Product Description |
Recombinant Canine LRRC15/LIB Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr47-Gly565. |
Product Background |
The chimeric protein binds in vitro and in vivo to a specific element upstream of LRRC15, leading to dramatic transcriptional activation.- LRRC15 encodes a leucine-rich transmembrane protein, present at the leading edge of migrating cells, the expression of which in normal tissues is restricted to the invasive cytotrophoblast layer of the placenta; small interfering (siRNA)-mediated suppression of LRRC15 expression in breast cancer cells leads to abrogation of invasiveness in vitro. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Canine LRRC15/LIB Protein (LRR-DM415) Specifications/Details
Protein |
LRRC15/LIB |
Synonyms |
LIB; LRRC15 |
Accession |
|
Species |
Canine |
Biotinylated |
no |
Amino Acid Range |
Tyr47-Gly565 |
Molecular Weight |
The protein has a predicted MW of 59.32 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Canine LRRC15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Canine LRRC15 is greater than 95% as determined by SEC-HPLC. |
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