Cynomolgus ALCAM/CD166 Protein (ALC-CM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus ALCAM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.22ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Trp28-Lys527

Catalog: ALC-CM101

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Product Description	Recombinant Cynomolgus ALCAM/CD166 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp28-Lys527.
Product Background	Brain metastasis (BM) in non-small-cell lung cancer (NSCLC) has a very poor prognosis. Recent studies have demonstrated the importance of cell adhesion molecules in tumor metastasis.Elevated levels of ALCAM expression promote BM formation in NSCLC through increased tumor cell dissemination and interaction with the brain endothelial cells. Therefore, ALCAM could be targeted to reduce the occurrence of BM.
Product Category	Recombinant Protein

Target	ALCAM/CD166
Gene/Protein ID	G7NZQ8
Species	Cynomolgus
Exact Sequence	Trp28-Lys527
Molecular Weight	The protein has a predicted MW of 57.10 kDa. Due to glycosylation, the protein migrates to 70-85 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus ALCAM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.22ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Cynomolgus ALCAM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.22ug/ml determined by ELISA.
	The purity of Cynomolgus ALCAM is greater than 95% as determined by SEC-HPLC.
	Cynomolgus ALCAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.