Cynomolgus B7-H3/CD276 Protein (BH7-CM173)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus B7-H3, His Tag at 0.5_g/ml (100_l/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu29-Glu465

Catalog: BH7-CM173

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Product Description Recombinant Cynomolgus B7-H3/CD276 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Leu29-Glu465.
Product Category Recombinant Protein
Target B7-H3/CD276
Synonyms B7H3; B7-H3; CD276; PSEC0249;ÊUNQ309; PRO352; B7 homolog 3; CD276
Gene/Protein ID XP_015308534.1
Species Cynomolgus
Exact Sequence Leu29-Glu465
Molecular Weight The protein has a predicted MW of 48.2 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Activity Immobilized Cynomolgus B7-H3, His Tag at 0.5_g/ml (100_l/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus B7-H3, His Tag at 0.5_g/ml (100_l/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA.
The purity of Cynomolgus B7-H3 is greater than 95% as determined by SEC-HPLC.
Cynomolgus B7-H3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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