Cynomolgus B7-H3/CD276 Protein (BH7-CM173)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus B7-H3, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu29-Glu465

Catalog: BH7-CM173

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Product Description Recombinant Cynomolgus B7-H3/CD276 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu29-Glu465.

Product Background

B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.

Product Category

Recombinant Protein

Target

B7-H3/CD276
Synonyms B7H3; B7-H3; CD276; PSEC0249;-UNQ309; PRO352; B7 homolog 3; CD276

Gene/Protein ID

XP_015308534.1

Species

Cynomolgus

Exact Sequence

Leu29-Glu465
Molecular Weight The protein has a predicted MW of 48.2 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Cynomolgus B7-H3, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus B7-H3, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA.
The purity of Cynomolgus B7-H3 is greater than 95% as determined by SEC-HPLC.
Cynomolgus B7-H3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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