Cynomolgus BTN3A1/CD277 Protein (BTN-CM4A1)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus BTN3A1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln1-Trp219

Catalog: BTN-CM4A1

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Product Description Recombinant Cynomolgus BTN3A1/CD277 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln1-Trp219.

Product Background

The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. BTN3A1 controls activation of human Vγ9/Vδ2 T cells by direct or indirect presentation of self and nonself phosphoantigens (pAg).

Product Category

Recombinant Protein

Target

BTN3A1/CD277
Synonyms CD277; BTN3A1; BTF5; BT3.1; BTN3.1
Accession

Species

Cynomolgus

Exact Sequence

Gln1-Trp219
Molecular Weight The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Cynomolgus BTN3A1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus BTN3A1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA.
The purity of Cynomolgus BTN3A1 is greater than 95% as determined by SEC-HPLC.
Cynomolgus BTN3A1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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