Non-biotinylated Cynomolgus BTN3A1/CD277 Protein (BTN-CM4A1)
Non-biotinylated Cynomolgus BTN3A1/CD277 Protein (BTN-CM4A1)
Non-biotinylated Cynomolgus BTN3A1/CD277 Protein (BTN-CM4A1)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus BTN3A1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln1-Trp219
Catalog #BTN-CM4A1
Regular price $346.50
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Non-biotinylated Cynomolgus BTN3A1/CD277 Protein (BTN-CM4A1) Summary

Product Description

Recombinant Cynomolgus BTN3A1/CD277 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln1-Trp219.

Product Background

The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. BTN3A1 controls activation of human Vγ9/Vδ2 T cells by direct or indirect presentation of self and nonself phosphoantigens (pAg).

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Non-biotinylated Cynomolgus BTN3A1/CD277 Protein (BTN-CM4A1) Specifications/Details

Protein
BTN3A1/CD277
Synonyms
CD277; BTN3A1; BTF5; BT3.1; BTN3.1
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Gln1-Trp219
Molecular Weight
The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus BTN3A1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Cynomolgus BTN3A1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA. Immobilized Cynomolgus BTN3A1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA.
Cynomolgus BTN3A1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus BTN3A1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus BTN3A1 is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus BTN3A1 is greater than 95% as determined by SEC-HPLC.
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