Cynomolgus CA125/MUC16 Protein (MUC-CM126)

Immobilized Cynomolgus CA125, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus MSLN, hFc Tag (Cat. MSL-CM280) with the EC50 of 0.69ng/ml determined by ELISA.

Cynomolgus CA125 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Cynomolgus CA125 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus CA125, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus MSLN, hFc Tag (Cat. MSL-CM280) with the EC50 of 0.69ng/ml determined by ELISA.
Amino Acid Range: Pro11544-Pro12188

Catalog #MUC-CM126

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Cynomolgus CA125/MUC16 Protein (MUC-CM126) Summary

Product Description

Recombinant Cynomolgus CA125/MUC16 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Pro11544-Pro12188.

Product Background

MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy.Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.Binding to MSLN mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to MSLN.

Cynomolgus CA125/MUC16 Protein (MUC-CM126) Specifications/Details

Protein	CA125/MUC16
Synonyms	CA125; CA-125; CA125MUC-16; FLJ14303; MUC16;CA125MUC16
Accession	XP_045234963.1
Species	Cynomolgus
Biotinylated	no
Amino Acid Range	Pro11544-Pro12188
Molecular Weight	The protein has a predicted MW of 73.67 kDa. Due to glycosylation, the protein migrates to 110-130 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Cynomolgus CA125, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus MSLN, hFc Tag (Cat. MSL-CM280) with the EC50 of 0.69ng/ml determined by ELISA.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS, 8% trehalose (pH 7.4).
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Cynomolgus CA125, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus MSLN, hFc Tag (Cat. MSL-CM280) with the EC50 of 0.69ng/ml determined by ELISA.
	Cynomolgus CA125 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Cynomolgus CA125 is greater than 95% as determined by SEC-HPLC.