Cynomolgus CD73/NT5E Protein (CD7-CM173)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus CD73, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Trp27-Cys554

Catalog: CD7-CM173

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Product Description Recombinant Cynomolgus CD73/NT5E Protein is expressed from Expi293 with His tag at the C-terminal.It contains Trp27-Cys554.
Product Category Recombinant Protein
Target CD73/NT5E
Synonyms CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55'-nucleotidase; NT5; NTE; CALJA
Gene/Protein ID EHH53214.1
Species Cynomolgus
Exact Sequence Trp27-Cys554
Molecular Weight The protein has a predicted MW of 59.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity Immobilized Cynomolgus CD73, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus CD73, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA.
Measured by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate  (AMP). The specific activity is > 10000 pmol/min/μg.
The purity of Cynomolgus CD73 is greater than 95% as determined by SEC-HPLC.
Cynomolgus CD73 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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