Product Description |
Recombinant Cynomolgus CTLA-4/CD152 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala37-Ser160. |
Product Background |
CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses.CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation – a phenomenon which is particularly notable in cancers. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Protein |
CTLA-4/CD152 |
Synonyms |
CTLA4; CD152; ICOS; CELIAC3; GRD4; GSE; ALPS5; IDDM12 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Ala37-Ser160 |
Molecular Weight |
The protein has a predicted MW of 14.4 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus CTLA-4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus B7-1, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). The affinity constant of 9.90 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Cynomolgus B7-1, His Tag immobilized on CM5 Chip can bind Cynomolgus CTLA-4, His Tag with an affinity constant of 9.90 nM as determined in SPR assay (Biacore T200). |
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Immobilized Cynomolgus CTLA-4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Cynomolgus B7-1, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). |
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Immobilized Cynomolgus CTLA-4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-CTLA-4 Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA. |
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Cynomolgus CTLA-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus CTLA-4 is greater than 95% as determined by SEC-HPLC. |
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