Cynomolgus CTLA-4/CD152 Protein (CTL-CM141)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus CTLA-4, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-CTLA-4 Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA. The affinity constant of 9.90 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ala37-Ser160

Catalog: CTL-CM141

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Product Description Recombinant Cynomolgus CTLA-4/CD152 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Ala37-Ser160.
Product Category Recombinant Protein
Target CTLA-4/CD152
Synonyms CTLA4; CD152; ICOS; CELIAC3; GRD4; GSE; ALPS5; IDDM12
Gene/Protein ID G7PL88-1
Species Cynomolgus
Exact Sequence Ala37-Ser160
Molecular Weight The protein has a predicted MW of 14.4 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
Activity Immobilized Cynomolgus CTLA-4, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-CTLA-4 Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA. The affinity constant of 9.90 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cynomolgus B7-1, His Tag immobilized on CM5 Chip can bind Cynomolgus CTLA-4, His Tag with an affinity constant of 9.90 nM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus CTLA-4, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-CTLA-4 Antibody, hFc Tag with the EC50 of 11.2ng/ml determined by ELISA.
The purity of Cynomolgus CTLA-4 is greater than 95% as determined by SEC-HPLC.
Cynomolgus CTLA-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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