Cynomolgus FOLR2 Protein (FOL-CM1R2)
|Recombinant Cynomolgus FOLR2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln9-His187.
|FOLR1 and FOLR2 are equipped with cellular glycosylphosphatidylinositol (GPI) anchors. FOLR1 is secreted from epithelia with or without a micelle-encapsulated GPI-anchor into milk and other body fluids/secretions, e.g. semen where its interaction with spermatozoa indicates a role in male fertility. FOLR1 and FOLR2 serve as serum biomarkers of various diseases. FOLR3 possesses no GPI-anchor and originates from secretory granules of neutrophil granulocytes; its concentration in serum correlates to the FOLR3 content in leukocytes and rises with increased leukocyte counts (infection, malignancy and pregnancy).
|The protein has a predicted MW of 25.5 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Tris-Bis PAGE result.
|Immobilized Cynomolgus FOLR2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FOLR2 Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA. See testing image for detail.
|> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
|Less than 1EU per ug by the LAL method.
|Shipped at ambient temperature.
|Lyophilized from 0.22um filtered solution in 50mM Tris,100mM Glycine (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
|Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
|Stability And Storage
|Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.