Product Description |
Recombinant Cynomolgus FOLR2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln9-His187. |
Product Background |
FOLR1 and FOLR2 are equipped with cellular glycosylphosphatidylinositol (GPI) anchors. FOLR1 is secreted from epithelia with or without a micelle-encapsulated GPI-anchor into milk and other body fluids/secretions, e.g. semen where its interaction with spermatozoa indicates a role in male fertility. FOLR1 and FOLR2 serve as serum biomarkers of various diseases. FOLR3 possesses no GPI-anchor and originates from secretory granules of neutrophil granulocytes; its concentration in serum correlates to the FOLR3 content in leukocytes and rises with increased leukocyte counts (infection, malignancy and pregnancy). |
Product Category |
Recombinant Protein |
Target |
FOLR2 |
Synonyms |
FBP；FOLR2；OLR2；BETA-HFR；FR-BETA；FR-P3 |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Gln9-His187 |
Molecular Weight |
The protein has a predicted MW of 25.5 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus FOLR2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FOLR2 Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 50mM Tris,100mM Glycine (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Cynomolgus FOLR2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-FOLR2 Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA. |
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Cynomolgus FOLR2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus FOLR2 is greater than 95% as determined by SEC-HPLC. |
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