Cynomolgus GPC3/Glypican 3 Protein (GPC-CM131)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus GPC3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 35.0ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gln25-His559

Catalog: GPC-CM131

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Product Description	Recombinant Cynomolgus GPC3/Glypican 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln25-His559.
Product Background	Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.
Product Category	Recombinant Protein

Target	GPC3/Glypican 3
Synonyms	GPC3; DGSX; Glypican 3; GTR2-2; MXR7; OCI5; OCI-5; SGB; SGBS; SGBS1SDYS; SDYS; SGBS1
Gene/Protein ID	XP_005594665.1
Species	Cynomolgus
Exact Sequence	Gln25-His559
Molecular Weight	The protein has a predicted MW of 61.8 kDa. Due to glycosylation, the protein migrates to 42 kDa, 70-135 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus GPC3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 35.0ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Cynomolgus GPC3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 35.0ng/ml determined by ELISA.
	The purity of Cynomolgus GPC3 is greater than 95% as determined by SEC-HPLC.
	Cynomolgus GPC3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.