Cynomolgus GUCY2C/Guanylyl cyclase C Protein (GCC-CM101)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus GUCY2C, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GUCY2C Antibody, hFc Tag with the EC50 of 103.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser24-Gln430

Catalog: GCC-CM101

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Product Description Recombinant Cynomolgus GUCY2C/Guanylyl cyclase C Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Ser24-Gln430.
Product Category Recombinant Protein
Target GUCY2C/Guanylyl cyclase C
Synonyms Guanylyl cyclase C; GC-C; STAR; GUCY2C; GUC2C; STA receptor; DIAR6; EC 4.6.1; GCC; GUC2CEC 4.6.1.2; MUCIL
Gene/Protein ID XP_005570270.1
Species Cynomolgus
Exact Sequence Ser24-Gln430
Molecular Weight The protein has a predicted MW of 47.2 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Tris-Bis PAGE result.
Activity Immobilized Cynomolgus GUCY2C, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GUCY2C Antibody, hFc Tag with the EC50 of 103.3ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus GUCY2C, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-GUCY2C Antibody, hFc Tag with the EC50 of 103.3ng/ml determined by ELISA.
The purity of Cynomolgus GUCY2C is greater than 95% as determined by SEC-HPLC.
Cynomolgus GUCY2C on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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