Cynomolgus IL-3 Protein (IL3-CE101)
  • Express System: E.coli
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 1.30 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Ala20-Gln143
Catalog #IL3-CE101
Regular price $346.50
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Size
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Cynomolgus IL-3 Protein (IL3-CE101) Summary

Product Description

Recombinant Cynomolgus IL-3 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ala20-Gln143.

Product Background

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Cynomolgus IL-3 Protein (IL3-CE101) Specifications/Details

Protein
IL-3
Synonyms
IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Ala20-Gln143
Molecular Weight
The protein has a predicted MW of 15.13 kDa same as Bis-Tris PAGE result.
Activity
The affinity constant of 1.30 nM as determined in SPR assay (Biacore T200).
Product Tag
N-His

Fluorophore

Unconjugated
Expression System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Cynomolgus IL-3 R alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-3, His Tag with an affinity constant of 1.30 nM as determined in SPR assay (Biacore T200). Cynomolgus IL-3 R alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-3, His Tag with an affinity constant of 1.30 nM as determined in SPR assay (Biacore T200).
Cynomolgus IL-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus IL-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus IL-3 is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus IL-3 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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