Product Description |
Recombinant Cynomolgus IL-3 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ala20-Gln143. |
Product Background |
Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
IL-3 |
Synonyms |
IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Ala20-Gln143 |
Molecular Weight |
The protein has a predicted MW of 15.13 kDa same as Bis-Tris PAGE result. |
Activity |
The affinity constant of 1.30 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
N-His |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Cynomolgus IL-3 R alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-3, His Tag with an affinity constant of 1.30 nM as determined in SPR assay (Biacore T200). |
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Cynomolgus IL-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus IL-3 is greater than 95% as determined by SEC-HPLC. |
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