Cynomolgus IL-3 Protein (IL3-CE101)
  • Express System: E.coli
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 1.30 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ala20-Gln143
Catalog #IL3-CE101
Regular price $315.00
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Product Description

Recombinant Cynomolgus IL-3 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ala20-Gln143.

Product Background

Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

Product Category

Recombinant Protein / Antibody discovery

Protein

IL-3

Synonyms

IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF

Accession

Species

Cynomolgus

Biotinylated

no

Amino Acid Range

Ala20-Gln143

Molecular Weight

The protein has a predicted MW of 15.13 kDa same as Bis-Tris PAGE result.

Activity

The affinity constant of 1.30 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

N-His

Expression System

E.coli

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Cynomolgus IL-3 R alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-3, His Tag with an affinity constant of 1.30 nM as determined in SPR assay (Biacore T200). Cynomolgus IL-3 R alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-3, His Tag with an affinity constant of 1.30 nM as determined in SPR assay (Biacore T200).
Cynomolgus IL-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus IL-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus IL-3 is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus IL-3 is greater than 95% as determined by SEC-HPLC.

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