Cynomolgus IL-3 R alpha/CD123 Protein (IL3-CM1RA)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.46 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Arg18-Arg302

Catalog: IL3-CM1RA

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Product Description Recombinant Cynomolgus IL-3 R alpha/CD123 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg18-Arg302.
Product Category Recombinant Protein
Target IL-3 R alpha/CD123
Synonyms CD123; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL3RAY; IL3RX; IL3RY; MGC34174; IL-3 R alpha
Gene/Protein ID G8F3K3-1
Species Cynomolgus
Exact Sequence Arg18-Arg302
Molecular Weight The protein has a predicted MW of 33.9 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 0.46 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-3, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus IL-3 R alpha, His Tag with an affinity constant of 0.46 uM as determined in SPR assay (Biacore T200) (QC Test).
Cynomolgus IL-3 R alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-3, His Tag with an affinity constant of 1.30 nM as determined in SPR assay (Biacore T200).
The purity of Cynomolgus IL-3 R alpha is greater than 95% as determined by SEC-HPLC.
Cynomolgus IL-3 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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