Cynomolgus MSLN/Mesothelin Protein (MSL-CM280)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CA125, His Tag at 5ug/ml (100ul/Well). Dose response curve for Cynomolgus MSLN, hFc Tag with the EC50 of 4.8ug/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Asp306-Gly590

Catalog: MSL-CM280

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Product Description	Recombinant Cynomolgus MSLN/Mesothelin Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Asp306-Gly590.
Product Background	Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.
Product Category	Recombinant Protein

Target	MSLN/Mesothelin
Synonyms	MSLN; CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP;Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein
Accession	XP_065393170.1
Species	Cynomolgus
Amino Acid Range	Asp306-Gly590
Molecular Weight	The protein has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CA125, His Tag at 5ug/ml (100ul/Well). Dose response curve for Cynomolgus MSLN, hFc Tag with the EC50 of 4.8ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CA125, His Tag at 5ug/ml (100ul/Well). Dose response curve for Cynomolgus MSLN, hFc Tag with the EC50 of 4.8ug/ml determined by ELISA.
	Immobilized Cynomolgus MSLN, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-MSLN Antibody, hFc Tag with the EC50 of 35.6ng/ml determined by ELISA (QC Test).
	The purity of Cynomolgus MSLN is greater than 95% as determined by SEC-HPLC.
	Cynomolgus MSLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.