Cynomolgus Mesothelin/MSLN Protein (MSL-CM280)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human CA125 at 5μg/ml (100μl/Well). Dose response curve for Cynomolgus MSLN, hFc Tag with the EC50 of 4.28μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp296-Gly580

Catalog: MSL-CM280

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Product Description Recombinant Cynomolgus Mesothelin/MSLN Protein is expressed from Expi293 with hFc tag at the C-terminal.; It contains Asp296-Gly580.
Product Category Recombinant Protein
Target MSLN/Mesothelin
Synonyms MSLN; CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein
Gene/Protein ID A0A2K5TW94-1
Species Cynomolgus
Exact Sequence Asp296-Gly580
Molecular Weight The protein has a predicted MW of 58.6 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human CA125 at 5μg/ml (100μl/Well). Dose response curve for Cynomolgus MSLN, hFc Tag with the EC50 of 4.28μg/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CA125 at 5μg/ml (100μl/Well). Dose response curve for Cynomolgus MSLN, hFc Tag with the EC50 of 4.28μg/ml determined by ELISA.
The purity of Cynomolgus MSLN is greater than 95% as determined by SEC-HPLC.
Cynomolgus MSLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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