Cynomolgus Nectin-4 Protein (NEC-CM104)
Cynomolgus Nectin-4 Protein (NEC-CM104)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus Nectin-4, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly32-Val351
Catalog #NEC-CM104
Regular price $315.00
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Product Description
Recombinant Cynomolgus Nectin-4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly32-Val351.
Product Background
Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.
Product Category
Recombinant Protein
Target
Nectin-4
Synonyms
EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Gly32-Val351
Molecular Weight
The protein has a predicted MW of 35.9 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus Nectin-4, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus Nectin-4, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.
Cynomolgus Nectin-4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Cynomolgus Nectin-4 is greater than 95% as determined by SEC-HPLC.

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