• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus PCSK9, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve Anti-PCSK9 Antibody, hFc Tag with the EC50 of 62.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.27 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Glu151-Gln811
Catalog #PCS-CM190
Price
Regular price $297.00
Regular price Sale price $297.00
/
Size
Avantor VWR Fisher Scientific

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Cynomolgus PCSK9 Protein (PCS-CM190) Summary

Product Description

 Recombinant Cynomolgus PCSK9 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu151-Gln811.

Product Background

 Proprotein convertase subtilisin/kexin type 9 (PCSK9) is an enzyme encoded by the PCSK9 gene in humans on chromosome 1.The first two PCSK9 inhibitors, alirocumab and evolocumab, were approved as once every two week injections, by the U.S. Food and Drug Administration in 2015 for lowering LDL-particle concentrations when statins and other drugs were not sufficiently effective or poorly tolerated.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Cynomolgus PCSK9 Protein (PCS-CM190) Specifications/Details

Protein
 PCSK9
Synonyms
 PCSK9
Accession
Species
 Cynomolgus
Biotinylated
 no
Amino Acid Range
 Glu151-Gln811
Molecular Weight
 Due to autocatalytic cleavage, the protein release the pro-form (59 kDa) and mature form (14 kDa). Due to glycosylation, the protein migrates to 65-70 kDa (pro-form) and 15-20 kDa (mature form) based on Bis-Tris PAGE result.
Activity
 Immobilized Cynomolgus PCSK9, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve Anti-PCSK9 Antibody, hFc Tag with the EC50 of 62.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.27 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Cynomolgus LDLR, His Tag immobilized on CM5 Chip can bind Cynomolgus PCSK9, His Tag with an affinity constant of 0.27 nM as determined in SPR assay (Biacore T200). Cynomolgus LDLR, His Tag immobilized on CM5 Chip can bind Cynomolgus PCSK9, His Tag with an affinity constant of 0.27 nM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus PCSK9, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve Anti-PCSK9 Antibody, hFc Tag with the EC50 of 62.0ng/ml determined by ELISA (QC Test). Immobilized Cynomolgus PCSK9, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve Anti-PCSK9 Antibody, hFc Tag with the EC50 of 62.0ng/ml determined by ELISA (QC Test).
Cynomolgus PCSK9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus PCSK9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus PCSK9 is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus PCSK9 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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