Cynomolgus/Rhesus macaque CD47 Protein (CD7-CM147)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus/Rhesus macaque CD47, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 0.52ug/ml determined by ELISA (QC Test). The affinity constant of 63.27 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln19-Glu141

Catalog: CD7-CM147

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Product Description Recombinant Cynomolgus/Rhesus macaque CD47 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Gln19-Glu141.
Product Category Recombinant Protein
Target CD47
Gene/Protein ID F7A802
Species Cynomolgus/Rhesus macaque
Exact Sequence Gln19-Glu141
Molecular Weight The protein has a predicted MW of 15 kDa. Due to glycosylation, the protein migrates to 45-65 kDa based on Tris-Bis PAGE result.
Activity Immobilized Cynomolgus/Rhesus macaque CD47, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 0.52ug/ml determined by ELISA (QC Test). The affinity constant of 63.27 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Cynomolgus SIRP alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus /Rhesus macaque CD47, His Tag with an affinity constant of 63.27 nM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus/Rhesus macaque CD47, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 0.52ug/ml determined by ELISA (QC Test).
The purity of Cynomolgus/Rhesus macaque CD47 is greater than 95% as determined by SEC-HPLC.
Cynomolgus/Rhesus macaque CD47 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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