Product Description |
Recombinant Cynomolgus/Rhesus macaque CD47 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln19-Glu141. |
Product Background |
CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers. |
Product Category |
Recombinant Protein |
Protein |
CD47 |
Synonyms |
CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6 |
Accession |
|
Species |
Cynomolgus/Rhesus macaque |
Biotinylated |
no |
Amino Acid Range |
Gln19-Glu141 |
Molecular Weight |
The protein has a predicted MW of 15 kDa. Due to glycosylation, the protein migrates to 45-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus/Rhesus macaque CD47, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA (QC Test). The affinity constant of 63.27 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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|
Cynomolgus SIRP alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus /Rhesus macaque CD47, His Tag with an affinity constant of 63.27 nM as determined in SPR assay (Biacore T200). |
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Immobilized Cynomolgus/Rhesus macaque CD47, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA (QC Test). |
|
|
Cynomolgus/Rhesus macaque CD47 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus/Rhesus macaque CD47 is greater than 95% as determined by SEC-HPLC. |
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