Cynomolgus TNF alpha Protein (TNF-CM10A)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus TNF alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 1.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.23 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Val77-Leu233

Catalog: TNF-CM10A

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Product Description	Recombinant Cynomolgus TNF alpha Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Val77-Leu233.
Product Background	Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Product Category	Recombinant Protein

Target	TNF alpha
Gene/Protein ID	P79337
Species	Cynomolgus
Exact Sequence	Val77-Leu233
Molecular Weight	The protein has a predicted MW of 18.35 kDa. Due to glycosylation, the protein migrates to 19-25 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Cynomolgus TNF alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 1.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.23 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Cynomolgus TNFR1, His Tag immobilized on CM5 Chip can bind Cynomolgus TNF alpha, His Tag with an affinity constant of 0.23 nM as determined in SPR assay (Biacore T200).
	Immobilized Cynomolgus TNF alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 1.4ng/ml determined by ELISA (QC Test).
	The purity of Cynomolgus TNF alpha is greater than 95% as determined by SEC-HPLC.
	Cynomolgus TNF alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.