Product Description |
Recombinant Cynomolgus TNF alpha Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Val77-Leu233. |
Product Background |
Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. |
Product Category |
Recombinant Protein |
Target |
TNF alpha |
Synonyms |
APC1 protein; Cachectin; DIF; TNF; TNFalpha; TNFATNF; TNFSF1A; TNFSF2; TNFA; TNFα; DIF; TNFSF2;TNF-alpha |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Val77-Leu233 |
Molecular Weight |
The protein has a predicted MW of 18.35 kDa. Due to glycosylation, the protein migrates to 19-25 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus TNF alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 1.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.23 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
N-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Cynomolgus TNFR1, His Tag immobilized on CM5 Chip can bind Cynomolgus TNF alpha, His Tag with an affinity constant of 0.23 nM as determined in SPR assay (Biacore T200). |
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Immobilized Cynomolgus TNF alpha, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR1, hFc Tag with the EC50 of 1.4ng/ml determined by ELISA (QC Test). |
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Cynomolgus TNF alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Cynomolgus TNF alpha is greater than 95% as determined by SEC-HPLC. |
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