Cynomolgus TPBG/5T4 Protein (5T4-CM101)
Cynomolgus TPBG/5T4 Protein (5T4-CM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus TPBG, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Thr35-Ser355
Catalog #5T4-CM101
Regular price $315.00
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Product Description
Recombinant Cynomolgus TPBG/5T4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr35-Ser355.
Product Background
The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs.
Product Category
Recombinant Protein
Target
TPBG/5T4
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Thr35-Ser355
Molecular Weight
The protein has a predicted MW of 35.95 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus TPBG, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus TPBG, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA.
Cynomolgus TPBG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus TPBG is greater than 95% as determined by SEC-HPLC.

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