Ferret TNF alpha-Protein (TNF-FE00A)
  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Ferret TNF-alpha, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, hFc Tag with the EC50 of 14.5ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Val77-Leu233
Catalog #TNF-FE00A
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Product Description
 Recombinant Ferret TNF alpha-Protein is expressed from E.coli without tag.It contains Val77-Leu233.
Product Background
 Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Product Category
 Recombinant Protein
Target
 TNF alpha
Accession
Species
 Ferret
Biotinylated
 no
Amino Acid Range
 Val77-Leu233
Molecular Weight
 The protein has a predicted MW of 17.21 kDa same as Bis-Tris PAGE result.
Activity
 Immobilized Ferret TNF-alpha, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, hFc Tag with the EC50 of 14.5ng/ml determined by ELISA. See testing image for detail.
Product Tag
 No Tag
Express System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Ferret TNF-alpha, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, hFc Tag with the EC50 of 14.5ng/ml determined by ELISA.
Ferret TNF alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Ferret TNF alpha is greater than 95% as determined by SEC-HPLC.
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