Ferret TNF alpha-Protein (TNF-FE10A)
Ferret TNF alpha-Protein (TNF-FE10A)
  • Express System: E.coli
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Ferret TNF-alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Val77-Leu233
Catalog #TNF-FE10A
Regular price $315.00
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Product Description
Recombinant Ferret TNF alpha-Protein is expressed from E.coli with His tag at the C-Terminus.It contains Val77-Leu233.
Product Background
Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Product Category
Recombinant Protein
Target
TNF alpha
Accession
Species
Ferret
Biotinylated
no
Amino Acid Range
Val77-Leu233
Molecular Weight
The protein has a predicted MW of 18.88 kDa same as Bis-Tris PAGE result.
Activity
Immobilized Ferret TNF-alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-His
Express System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Ferret TNF-alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test).
Ferret TNF alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Ferret TNF alpha is greater than 95% as determined by SEC-HPLC.

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