Name: Ferret TNF alpha-Protein (TNF-FE10A)
Brand: KactusBio
SKU: TNF-FE10A-100UG
Price: 346.5 USD
Availability: InStock

Express System: E.coli
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Ferret TNF-alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Val77-Leu233

Catalog #TNF-FE10A

Size

100UG

500UG

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Ferret TNF alpha-Protein (TNF-FE10A) Summary

Product Description	Recombinant Ferret TNF alpha-Protein is expressed from E.coli with His tag at the C-Terminus.It contains Val77-Leu233.
Product Background	Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Ferret TNF alpha-Protein (TNF-FE10A) Specifications/Details

Protein	TNF alpha
Synonyms	APC1 protein; Cachectin; DIF; TNF; TNFalpha; TNFATNF; TNFSF1A; TNFSF2; TNFA; TNFα; DIF; TNFSF2;TNF-alpha
Accession	A3FBF1
Species	Ferret
Biotinylated	no
Amino Acid Range	Val77-Leu233
Molecular Weight	The protein has a predicted MW of 18.88 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Ferret TNF-alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Ferret TNF-alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test).
	Ferret TNF alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Ferret TNF alpha is greater than 95% as determined by SEC-HPLC.