FITC-Labeled Human CD47 Protein (CD7-HM147F)

Immobilized FITC-Labeled Human CD47, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 77.4ng/ml determined by ELISA (QC Test).

FITC-Labeled Human CD47 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of FITC-Labeled Human CD47 is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized FITC-Labeled Human CD47, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 77.4ng/ml determined by ELISA (QC Test).
Amino Acid Range: Gln19-Pro139

Catalog #CD7-HM147F

Price

$990⁰⁰

Size

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FITC-Labeled Human CD47 Protein (CD7-HM147F) Summary

Product Description

Recombinant FITC-Labeled Human CD47 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln19-Pro139.

Product Background

CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.

FITC-Labeled Human CD47 Protein (CD7-HM147F) Specifications/Details

Protein	CD47
Synonyms	CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6
Accession	Q08722-1
Species	Human
Biotinylated	no
Amino Acid Range	Gln19-Pro139
Molecular Weight	The protein has a predicted MW of 14.5 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.
Activity	Immobilized FITC-Labeled Human CD47, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 77.4ng/ml determined by ELISA (QC Test).
Product Tag	C-His
Fluorophore	FITC-Labeled
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized FITC-Labeled Human CD47, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 77.4ng/ml determined by ELISA (QC Test).
	FITC-Labeled Human CD47 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of FITC-Labeled Human CD47 is greater than 90% as determined by SEC-HPLC.