FITC-Labeled Human CD47 Protein (CD7-HM147F)
FITC-Labeled Human CD47 Protein (CD7-HM147F)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized FITC-Labeled Human CD47, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 77.4ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gln19-Pro139
Catalog #CD7-HM147F
Regular price $900.00
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Product Description
Recombinant FITC-Labeled Human CD47 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Gln19-Pro139.
Product Background
CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.
Product Category
Recombinant Protein
Target
CD47
Synonyms
CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln19-Pro139
Molecular Weight
The protein has a predicted MW of 14.5 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.
Activity
Immobilized FITC-Labeled Human CD47, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 77.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-His
Fluorophore
FITC
Express System
HEK293
Purity
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 6 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized FITC-Labeled Human CD47, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 77.4ng/ml determined by ELISA (QC Test).
FITC-Labeled Human CD47 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of FITC-Labeled Human CD47 is greater than 95% as determined by SEC-HPLC.

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