FITC-Labeled Human EGFRVIII Protein (EG8-HM154F)
Catalog: EG8-HM154F
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| Product Description | Recombinant FITC-Labeled Human EGFRVIII Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Leu25-Ser378. |
| Product Category | Recombinant Protein |
| Target | EGFRVIII |
| Synonyms | ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2 |
| Gene/Protein ID | NP_001333870.1 |
| Species | Human |
| Exact Sequence | Leu25-Ser378 |
| Molecular Weight | The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Liquid |
| Shipping | Shipped with blue ice or dry ice. |
| Formulation | Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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FACS Analysis of Anti-EGFRVIII CAR Expression. 293T cells were transfected with anti-EGFRVIII-scFv and His tag. Cells were incubated with FITC-Labeled Human EGFRVIII, His Tag and FITC-labeled protein control. Non-transfected 293T cells and FITC-labeled protein control were used as negative control. |
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FITC-Labeled Human EGFRVIII on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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