Name: FITC-Labeled Human EGFRVIII Protein (EG8-HM154F)
Brand: KactusBio
SKU: EG8-HM154F-100UG
Price: 990.0 USD
Availability: InStock

FITC-Labeled Human EGFRVIII Protein (EG8-HM154F)

Immobilized FITC-Labeled Human EGFRVIII, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EGFRVIII Antibody, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA (QC Test).

FITC-Labeled Human EGFRVIII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

FACS Analysis of Anti-EGFRVIII CAR Expression. 293T cells were transfected with anti-EGFRVIII-scFv and His tag. Cells were incubated with 5ug/ml FITC-Labeled Human EGFRVIII, His Tag and FITC-labeled protein control. Non-transfected 293T cells and FITC-labeled protein control were used as negative control.

The purity of FITC-Labeled Human EGFRVIII is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized FITC-Labeled Human EGFRVIII, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EGFRVIII Antibody, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Leu25-Ser378

Catalog #EG8-HM154F

Size

100UG

500UG

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Product Description	Recombinant FITC-Labeled Human EGFRVIII Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu25-Ser378.
Product Background	The epidermal growth factor receptor (EGFR) is overexpressed in a variety of human epithelial tumors, often as a consequence of gene amplification. Tumors with EGFR gene amplification frequently contain EGFR gene rearrangements, with the most common extracellular domain mutation being EGFRvIII. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Protein	EGFRVIII
Synonyms	ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2
Accession	NP_001333870.1
Species	Human
Biotinylated	no
Amino Acid Range	Leu25-Ser378
Molecular Weight	The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized FITC-Labeled Human EGFRVIII, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EGFRVIII Antibody, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22um filtered solution in PBS (pH 7.4).
Stability And Storage	Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized FITC-Labeled Human EGFRVIII, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-EGFRVIII Antibody, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA (QC Test).
	FITC-Labeled Human EGFRVIII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	FACS Analysis of Anti-EGFRVIII CAR Expression. 293T cells were transfected with anti-EGFRVIII-scFv and His tag. Cells were incubated with 5ug/ml FITC-Labeled Human EGFRVIII, His Tag and FITC-labeled protein control. Non-transfected 293T cells and FITC-labeled protein control were used as negative control.
	The purity of FITC-Labeled Human EGFRVIII is greater than 95% as determined by SEC-HPLC.