FITC-Labeled Human Her3/ErbB3 Protein (HER-HM403F)
|Recombinant FITC-Labeled Human Her3/ErbB3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser20-Thr643.
|Her3, also called ErbB3, is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors.Her3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric Her3 serves as a low affinity receptor for the heregulins (HRG).
|The protein has a predicted MW of 71.6 kDa. Due to glycosylation, the protein migrates to 72-75 kDa based on Tris-Bis PAGE result.
|Immobilized FITC-Labeled Human Her3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human NRG1 Beta 1, hFc Tag with the EC50 of 23.6ng/ml determined by ELISA. See testing image for detail.
|> 95% as determined by Tris-Bis PAGE
|Less than 1EU per ug by the LAL method.
|Shipped at ambient temperature.
|Lyophilized from 0.22 um filtered solution in HER-HM403F. Normally 8% trehalose is added as protectant before lyophilization.
|Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
|Stability And Storage
|Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.