FITC-Labeled Human MSLN/Mesothelin Protein (MSL-HM480F)
Catalog: MSL-HM480F
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| Product Description | Recombinant FITC-Labeled Human MSLN/Mesothelin Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu296-Gly580. |
Product Background |
Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally. |
Product Category |
Recombinant Protein |
Target |
MSLN/Mesothelin |
| Synonyms | MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR |
Gene/Protein ID |
Q13421-2 |
Species |
Human |
Exact Sequence |
Glu296-Gly580 |
| Molecular Weight | The protein has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Liquid |
| Shipping | Shipped with dry ice. |
| Formulation | Supplied as 0.22um filtered solution in PBS (pH 7.4). |
| Stability And Storage | Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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FACS Analysis of Anti-MSLN CAR Expression. 293T cells were transfected with anti-MSLN-scFv and His tag. Cells were stained with 5ug/ml FITC-Labeled Human MSLN (296-580) , His Tag and FITC-labeled protein control. Non-transfected 293T cells and FITC-labeled protein control were used as negative control. |
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FITC-Labeled Human MSLN/Mesothelin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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