- Express System: HEK293
- Product Tag: C-His-Avi
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized FITC-Labeled Human TPBG, His Tag at 0.5ug/ml(100ul/well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA (QC Test).
- Amino Acid Range: Ser32-Ser355
FITC-Labeled Human TPBG/5T4 Protein (5T4-HM401F) Summary
Product Description |
Recombinant FITC-Labeled Human TPBG/5T4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser32-Ser355. |
Product Background |
The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets |
FITC-Labeled Human TPBG/5T4 Protein (5T4-HM401F) Specifications/Details
Protein |
TPBG/5T4 |
Synonyms |
M6P1; 5T4AG; WAIF1; TPBG; 5T4 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser32-Ser355 |
Molecular Weight |
The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized FITC-Labeled Human TPBG, His Tag at 0.5ug/ml(100ul/well) on the plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 6.4ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His-Avi |
Fluorophore |
FITC |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22um filtered solution in PBS (pH 7.4). |
Stability And Storage |
Valid for 6 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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