Product Description |
Recombinant Human 2B4/CD244/SLAMF4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Cys22-Arg221. |
Product Background |
2B4 (CD244) is expressed by memory-phenotype CD8() T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness. |
Product Category |
Recombinant Protein |
Target |
2B4/CD244/SLAMF4 |
Synonyms |
2B4; CD244 antigen; CD244; h2B4; NAIL; NKR2B4; Nmrk; SLAMF4 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Cys22-Arg221 |
Molecular Weight |
The protein has a predicted MW of 49 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD48, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human 2B4, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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|
Immobilized Human CD48, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human 2B4, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA. |
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Human 2B4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human 2B4 is greater than 95% as determined by SEC-HPLC. |
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