Human 2B4/CD244/SLAMF4 Protein (2B4-HM201)
Catalog: 2B4-HM201
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| Product Description | Recombinant Human 2B4/CD244/SLAMF4 Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Cys22-Arg221. |
| Product Category | Recombinant Protein |
| Target | 2B4/CD244/SLAMF4 |
| Synonyms | 2B4; CD244 antigen; CD244; h2B4; NAIL; NKR2B4; Nmrk; SLAMF4 |
| Gene/Protein ID | Q9BZW8-2 |
| Species | Human |
| Exact Sequence | Cys22-Arg221 |
| Molecular Weight | The protein has a predicted MW of 49 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human CD48, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human 2B4, hFc Tag with the EC50 of 0.45μg/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human CD48, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human 2B4, hFc Tag with the EC50 of 0.45μg/ml determined by ELISA. |
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The purity of Human 2B4/CD244/SLAMF4 is greater than 95% as determined by SEC-HPLC. |
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Human 2B4/CD244/SLAMF4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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