Human 2B4/CD244/SLAMF4 Protein (2B4-HM201)
Human 2B4/CD244/SLAMF4 Protein (2B4-HM201)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD48, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human 2B4, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Cys22-Arg221
Catalog #2B4-HM201
Regular price $223.30
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Product Description

Recombinant Human 2B4/CD244/SLAMF4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Cys22-Arg221.

Product Background

2B4 (CD244) is expressed by memory-phenotype CD8(+) T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

2B4/CD244/SLAMF4

Synonyms

2B4; CD244 antigen; CD244; h2B4; NAIL; NKR2B4; Nmrk; SLAMF4

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Cys22-Arg221

Molecular Weight

The protein has a predicted MW of 49 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human CD48, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human 2B4, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 20mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human CD48, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human 2B4, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA. Immobilized Human CD48, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human 2B4, hFc Tag with the EC50 of 0.13ug/ml determined by ELISA.
Human 2B4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human 2B4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human 2B4 is greater than 95% as determined by SEC-HPLC. The purity of Human 2B4 is greater than 95% as determined by SEC-HPLC.

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