Human ALCAM/CD166 Protein (ALC-HM101)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ALCAM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.26ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Trp28-Ala526

Catalog: ALC-HM101

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Product Description Recombinant Human ALCAM/CD166 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp28-Ala526.
Product Category Recombinant Protein
Target ALCAM/CD166
Synonyms CD166; ALCAM; MEMD; CD6L; KG-CAM
Gene/Protein ID Q13740-1
Species Human
Exact Sequence Trp28-Ala526
Molecular Weight The protein has a predicted MW of 57 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human ALCAM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.26ug/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human ALCAM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.26ug/ml determined by ELISA.
The purity of Human ALCAM is greater than 95% as determined by SEC-HPLC.
Human ALCAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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