Human ALCAM/CD166 Protein (ALC-HM101)
Human ALCAM/CD166 Protein (ALC-HM101)
Human ALCAM/CD166 Protein (ALC-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ALCAM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.28ug/ml determined by ELISA (QC Test).
  • Amino Acid Range: Trp28-Ala526
Catalog #ALC-HM101
Regular price $132.00
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Size
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Human ALCAM/CD166 Protein (ALC-HM101) Summary

Product Description

Recombinant Human ALCAM/CD166 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp28-Ala526.

Product Background

Activated Leukocyte Cell Adhesion Molecule (ALCAM/CD166) is a glycoprotein involved in homotypic and heterotypic cell adhesion. ALCAM can be proteolytically cleaved at the cell surface by metalloproteases, which generate shedding of its ectodomain. In various tumors, ALCAM is overexpressed and serves as a valuable prognostic marker of disease progression.

Product Category

Recombinant Protein / Antibody discovery / ADC targets

Human ALCAM/CD166 Protein (ALC-HM101) Specifications/Details

Protein
ALCAM/CD166
Synonyms
CD166; ALCAM; MEMD; CD6L; KG-CAM
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Trp28-Ala526
Molecular Weight
The protein has a predicted MW of 57 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human ALCAM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.28ug/ml determined by ELISA (QC Test).
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human ALCAM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.28ug/ml determined by ELISA (QC Test). Immobilized Human ALCAM, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.28ug/ml determined by ELISA (QC Test).
Human ALCAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human ALCAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human ALCAM is greater than 95% as determined by SEC-HPLC. The purity of Human ALCAM is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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