Human ALCAM/CD166 Protein (ALC-HM201)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human ALCAM, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.3ug/ml determined by ELISA (QC Test). The affinity constant of 0.53 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Trp28-Ala526

Catalog: ALC-HM201

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Product Description	Recombinant Human ALCAM/CD166 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Trp28-Ala526.
Product Background	Brain metastasis (BM) in non-small-cell lung cancer (NSCLC) has a very poor prognosis. Recent studies have demonstrated the importance of cell adhesion molecules in tumor metastasis.Elevated levels of ALCAM expression promote BM formation in NSCLC through increased tumor cell dissemination and interaction with the brain endothelial cells. Therefore, ALCAM could be targeted to reduce the occurrence of BM.
Product Category	Recombinant Protein

Target	ALCAM/CD166
Synonyms	CD166; ALCAM; MEMD; CD6L; KG-CAM
Gene/Protein ID	Q13740-1
Species	Human
Exact Sequence	Trp28-Ala526
Molecular Weight	The protein has a predicted MW of 82.7 kDa. Due to glycosylation, the protein migrates to 90-115 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human ALCAM, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.3ug/ml determined by ELISA (QC Test). The affinity constant of 0.53 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human ALCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD6, His Tag with an affinity constant of 0.53 uM as determined in SPR assay (Biacore T200).
	Immobilized Human ALCAM, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.3ug/ml determined by ELISA (QC Test).
	Immobilized Human CD6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human ALCAM, hFc Tag with the EC50 of 10.7ng/ml determined by ELISA.
	The purity of Human ALCAM is greater than 95% as determined by SEC-HPLC.
	Human ALCAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.