Product Description |
Recombinant Human ALCAM/CD166 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Trp28-Ala526. |
Product Background |
Brain metastasis (BM) in non-small-cell lung cancer (NSCLC) has a very poor prognosis. Recent studies have demonstrated the importance of cell adhesion molecules in tumor metastasis.Elevated levels of ALCAM expression promote BM formation in NSCLC through increased tumor cell dissemination and interaction with the brain endothelial cells. Therefore, ALCAM could be targeted to reduce the occurrence of BM. |
Product Category |
Recombinant Protein |
Target |
ALCAM/CD166 |
Synonyms |
CD166; ALCAM; MEMD; CD6L; KG-CAM |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Trp28-Ala526 |
Molecular Weight |
The protein has a predicted MW of 82.7 kDa. Due to glycosylation, the protein migrates to 90-115 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human ALCAM, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.3ug/ml determined by ELISA. The affinity constant of 0.53 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human ALCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD6, His Tag with an affinity constant of 0.53 uM as determined in SPR assay (Biacore T200). |
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Immobilized Human ALCAM, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.3ug/ml determined by ELISA. |
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Immobilized Human CD6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human ALCAM, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). |
|
|
Human ALCAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human ALCAM is greater than 95% as determined by SEC-HPLC. |
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