Human ALCAM/CD166 Protein (ALC-HM201)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human ALCAM, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.3ug/ml determined by ELISA (QC Test). The affinity constant of 0.53 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Trp28-Ala526

Catalog: ALC-HM201

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Product Description Recombinant Human ALCAM/CD166 Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Trp28-Ala526.
Product Category Recombinant Protein
Target ALCAM/CD166
Synonyms CD166; ALCAM; MEMD; CD6L; KG-CAM
Gene/Protein ID Q13740-1
Species Human
Exact Sequence Trp28-Ala526
Molecular Weight The protein has a predicted MW of 82.7 kDa. Due to glycosylation, the protein migrates to 90-115 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human ALCAM, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.3ug/ml determined by ELISA (QC Test). The affinity constant of 0.53 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human ALCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD6, His Tag with an affinity constant of 0.53 μM as determined in SPR assay (Biacore T200).
Immobilized Human ALCAM, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-ALCAM Antibody, hFc Tag with the EC50 of 0.3ug/ml determined by ELISA (QC Test).
The purity of Human ALCAM is greater than 95% as determined by SEC-HPLC.
Human ALCAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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