Human AXL Protein (AXL-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human AXL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Glu33-Ser442

Catalog: AXL-HM101

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Product Description	Recombinant Human AXL Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu33-Ser442.
Product Background	Axl, a member of the TAM (Tyro3, Axl, Mer) family, and its inhibitors can specifically break the kinase signaling nodes, allowing advanced patients to regain drug sensitivity with improved therapeutic efficacy. Overexpression and activation of Axl receptor tyrosine kinase have been widely accepted to promote cell proliferation, chemotherapy resistance, invasion, and metastasis in several human cancers, such as lung, breast, and pancreatic cancers.
Product Category	Recombinant Protein

Target	AXL
Synonyms	Axl; UFO; AXL oncogene; AXL; ARK; JTK11; Tyro7; AI323647; EC 2.7.10; EC 2.7.10.1
Gene/Protein ID	NP_068713.2
Species	Human
Exact Sequence	Glu33-Ser442
Molecular Weight	The protein has a predicted MW of 45.4 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human AXL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human AXL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA.
	The purity of Human AXL is greater than 95% as determined by SEC-HPLC.
	Human AXL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.