Human AXL Protein (AXL-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human AXL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Glu33-Ser442
Catalog #AXL-HM101
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Product Description
 Recombinant Human AXL Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu33-Ser442.
Product Background
 Axl, a member of the TAM (Tyro3, Axl, Mer) family, and its inhibitors can specifically break the kinase signaling nodes, allowing advanced patients to regain drug sensitivity with improved therapeutic efficacy. Overexpression and activation of Axl receptor tyrosine kinase have been widely accepted to promote cell proliferation, chemotherapy resistance, invasion, and metastasis in several human cancers, such as lung, breast, and pancreatic cancers.
Product Category
 Recombinant Protein
Target
 AXL
Synonyms
 Axl; UFO; AXL oncogene; AXL; ARK; JTK11; Tyro7; AI323647; EC 2.7.10; EC 2.7.10.1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Glu33-Ser442
Molecular Weight
 The protein has a predicted MW of 45.4 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human AXL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human AXL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-AXL Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA.
Human AXL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human AXL is greater than 95% as determined by SEC-HPLC.
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