Name: Human B7-H3/CD276 Protein (BH7-HM173)
Brand: KactusBio
SKU: BH7-HM173-100UG
Price: 264.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: >95% as determined by Bis-Tris PAGE
Activity: Immobilized Human B7-H3, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 9.9 ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Leu29-Pro245

Catalog #BH7-HM173

Size

100UG

500UG

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Product Description	Recombinant Human B7-H3/CD276 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu29-Pro245.
Product Background	B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers. B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Protein	B7-H3/CD276
Synonyms	B7H3; B7-H3; CD276; PSEC0249;-UNQ309; PRO352; B7 homolog 3; CD276
Accession	Q5ZPR3-2
Species	Human
Biotinylated	no
Amino Acid Range	Leu29-Pro245
Molecular Weight	The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human B7-H3, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 9.9 ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	>95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human B7-H3, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 9.9 ng/ml determined by ELISA.
	Human B7-H3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.