Human B7-H3/CD276 Protein (BH7-HM173)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human B7-H3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 29.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu29-Pro245

Catalog: BH7-HM173

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Product Description Recombinant Human B7-H3/CD276 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Leu29-Pro245.
Product Category Recombinant Protein
Target B7-H3/CD276
Synonyms B7H3; B7-H3; CD276; PSEC0249; UNQ309; PRO352; B7 homolog 3; CD276
Gene/Protein ID Q5ZPR3-2
Species Human
Exact Sequence Leu29-Pro245
Molecular Weight The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human B7-H3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 29.4ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human B7-H3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-B7-H3 Antibody, hFc Tag with the EC50 of 29.4ng/ml determined by ELISA.
The purity of Human B7-H3 is greater than 95% as determined by SEC-HPLC.
Human B7-H3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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