Name: Human B7-H3/CD276 Protein (BH7-HM273)
Brand: KactusBio
SKU: BH7-HM273-100UG
Price: 264.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human B7-H3, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 61.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Leu29-Pro245

Catalog #BH7-HM273

Size

100UG

500UG

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Product Description	Recombinant Human B7-H3/CD276 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu29-Pro245.
Product Background	B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig‑B7‑H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Protein	B7-H3/CD276
Synonyms	B7H3; B7-H3; CD276; PSEC0249;-UNQ309; PRO352; B7 homolog 3; CD276
Accession	Q5ZPR3-2
Species	Human
Biotinylated	no
Amino Acid Range	Leu29-Pro245
Molecular Weight	The protein has a predicted MW of 50.1 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human B7-H3, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 61.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human B7-H3, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 61.0ng/ml determined by ELISA (QC Test).
	Human B7-H3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human B7-H3 is greater than 95% as determined by SEC-HPLC.