Product Description |
Recombinant Human BTN2A1&BTN3A1 complex Protein is expressed from HEK293 with His tag and Flag tag at the C-terminus.It contains Gln29-Ala248 (BTN2A1) acidic tail and Gln30-Gly254 (BTN3A1) basic tail. |
Product Background |
BTN2A1 and BTN3A1 clearly play highly distinct roles in Vγ9Vδ2 T cell detection of P-Ag-exposed cells. BTN2A1&BTN3A1, a protein complex which contains heterodimeric BTN2A1 and BTN3A1 extracellular domains (ECD), is critical to enhance cytotoxic killing by Vγ9Vδ2 T cells. |
Product Category |
Recombinant Protein |
Target |
BTN2A1&BTN3A1 complex |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln29-Ala248 (BTN2A1) acidic tail & Gln30-Gly254 (BTN3A1) basic tail |
Molecular Weight |
The protein has a predicted MW of 30.74 kDa (BTN2A1) and 30.25 kDa (BTN3A1). Due to glycosylation, the protein migrates to 53-63 kDa (BTN2A1) and 33-38 kDa (BTN3A1) based on Bis-Tris PAGE result. |
Activity |
Immobilized Human BTN3A1&BTN2A1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His, C-Flag |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human BTN3A1&BTN2A1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. |
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Human BTN2A1&BTN3A1 complex on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human BTN2A1&BTN3A1 complex is greater than 95% as determined by SEC-HPLC. |
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