Name: Human BTN2A1&BTN3A1 complex Protein (BTN-HM123)
Brand: KactusBio
SKU: BTN-HM123-100UG
Price: 375.0 USD
Availability: InStock

Human BTN2A1&BTN3A1 complex Protein (BTN-HM123)

Immobilized Human BTN3A1&BTN2A1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test).

Human BTN2A1&BTN3A1 complex on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human BTN2A1&BTN3A1 complex is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His, C-Flag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human BTN3A1&BTN2A1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gln29-Ala248 (BTN2A1) acidic tail & Gln30-Gly254 (BTN3A1) basic tail

Catalog #BTN-HM123

Size

100UG

500UG

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Product Description	Recombinant Human BTN2A1&BTN3A1 complex Protein is expressed from HEK293 with His tag and Flag tag at the C-terminus.It contains Gln29-Ala248 (BTN2A1) acidic tail and Gln30-Gly254 (BTN3A1) basic tail.
Product Background	BTN2A1 and BTN3A1 clearly play highly distinct roles in Vγ9Vδ2 T cell detection of P-Ag-exposed cells. BTN2A1&BTN3A1, a protein complex which contains heterodimeric BTN2A1 and BTN3A1 extracellular domains (ECD), is critical to enhance cytotoxic killing by Vγ9Vδ2 T cells.
Product Category	Recombinant Protein / Antibody discovery

Protein	BTN2A1&BTN3A1 complex
Synonyms	BTN3A1&BTN2A1; BTN2A1&BTN3A1;
Accession	Q7KYR7-1(BTN2A1)&O00481-1(BTN3A1)
Species	Human
Biotinylated	no
Amino Acid Range	Gln29-Ala248 (BTN2A1) acidic tail & Gln30-Gly254 (BTN3A1) basic tail
Molecular Weight	The protein has a predicted MW of 30.74 kDa (BTN2A1) and 30.25 kDa (BTN3A1). Due to glycosylation, the protein migrates to 53-63 kDa (BTN2A1) and 33-38 kDa (BTN3A1) based on Bis-Tris PAGE result.
Activity	Immobilized Human BTN3A1&BTN2A1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His, C-Flag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human BTN3A1&BTN2A1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test).
	Human BTN2A1&BTN3A1 complex on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human BTN2A1&BTN3A1 complex is greater than 95% as determined by SEC-HPLC.