Name: Human BTN2A1&BTN3A1 complex Protein (BTN-HM123)
Brand: KactusBio
SKU: BTN-HM123-100UG
Price: 412.5 USD
Availability: InStock

ELISA binding curve of Human BTN3A1 and BTN2A1 His Tag with increasing concentrations of anti-BTN3A1 hFc Tag antibody in a recombinant protein interaction assay.

Human BTN2A1&BTN3A1 complex Protein (BTN-HM123)

Immobilized Human BTN3A1&BTN2A1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test).

Human BTN2A1&BTN3A1 complex on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human BTN2A1&BTN3A1 complex is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His, C-Flag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human BTN3A1&BTN2A1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test).
Amino Acid Range: Gln29-Ala248 (BTN2A1) acidic tail & Gln30-Gly254 (BTN3A1) basic tail

Catalog #BTN-HM123

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human BTN2A1&BTN3A1 complex Protein (BTN-HM123) Summary

Product Description	Recombinant Human BTN2A1&BTN3A1 complex Protein is expressed from HEK293 with His tag at the C-terminus of BTN2A1 and Flag tag at the C-terminus of BTN3A1.It contains Gln29-Ala248 (BTN2A1) acidic tail and Gln30-Gly254 (BTN3A1) basic tail.
Product Background	BTN2A1 and BTN3A1 clearly play highly distinct roles in Vγ9Vδ2 T cell detection of P-Ag-exposed cells. BTN2A1&BTN3A1, a protein complex which contains heterodimeric BTN2A1 and BTN3A1 extracellular domains (ECD), is critical to enhance cytotoxic killing by Vγ9Vδ2 T cells.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human BTN2A1&BTN3A1 complex Protein (BTN-HM123) Specifications/Details

Protein	BTN2A1&BTN3A1
Synonyms	BTN3A1&BTN2A1; BTN2A1&BTN3A1;
Accession	Q7KYR7-1(BTN2A1)&O00481-1(BTN3A1)
Species	Human
Biotinylated	no
Amino Acid Range	Gln29-Ala248 (BTN2A1) acidic tail & Gln30-Gly254 (BTN3A1) basic tail
Molecular Weight	The protein has a predicted MW of 30.74 kDa (BTN2A1) and 30.25 kDa (BTN3A1). Due to glycosylation, the protein migrates to 53-63 kDa and 33-38 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human BTN3A1&BTN2A1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test).
Product Tag	C-His, C-Flag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human BTN3A1&BTN2A1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test).
	Human BTN2A1&BTN3A1 complex on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human BTN2A1&BTN3A1 complex is greater than 95% as determined by SEC-HPLC.