- Express System: HEK293
- Product Tag: C-His, C-Flag
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Human BTN3A1&BTN2A1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). See testing image for detail.
- Amino Acid Range: Gln29-Ala248 (BTN2A1) acidic tail & Gln30-Gly254 (BTN3A1) basic tail
Human BTN2A1&BTN3A1 complex Protein (BTN-HM123) Summary
Product Description |
Recombinant Human BTN2A1&BTN3A1 complex Protein is expressed from HEK293 with His tag at the C-terminus of BTN2A1 and Flag tag at the C-terminus of BTN3A1.It contains Gln29-Ala248 (BTN2A1) acidic tail and Gln30-Gly254 (BTN3A1) basic tail. |
Product Background |
BTN2A1 and BTN3A1 clearly play highly distinct roles in Vγ9Vδ2 T cell detection of P-Ag-exposed cells. BTN2A1&BTN3A1, a protein complex which contains heterodimeric BTN2A1 and BTN3A1 extracellular domains (ECD), is critical to enhance cytotoxic killing by Vγ9Vδ2 T cells. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human BTN2A1&BTN3A1 complex Protein (BTN-HM123) Specifications/Details
Protein |
BTN2A1&BTN3A1 |
Synonyms |
BTN3A1&BTN2A1; BTN2A1&BTN3A1; |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln29-Ala248 (BTN2A1) acidic tail & Gln30-Gly254 (BTN3A1) basic tail |
Molecular Weight |
The protein has a predicted MW of 30.74 kDa (BTN2A1) and 30.25 kDa (BTN3A1). Due to glycosylation, the protein migrates to 53-63 kDa and 33-38 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human BTN3A1&BTN2A1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His, C-Flag |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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