ELISA binding curve of Human BTN3A1 and BTN2A1 His Tag with increasing concentrations of anti-BTN3A1 hFc Tag antibody in a recombinant protein interaction assay.
Human BTN2A1&BTN3A1 complex Protein (BTN-HM123)
  • Express System: HEK293
  • Product Tag: C-His, C-Flag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human BTN3A1&BTN2A1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gln29-Ala248 (BTN2A1) acidic tail & Gln30-Gly254 (BTN3A1) basic tail
Catalog #BTN-HM123
Regular price $412.50
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Human BTN2A1&BTN3A1 complex Protein (BTN-HM123) Summary

Product Description

Recombinant Human BTN2A1&BTN3A1 complex Protein is expressed from HEK293 with His tag at the C-terminus of BTN2A1 and Flag tag at the C-terminus of BTN3A1.It contains Gln29-Ala248 (BTN2A1) acidic tail and Gln30-Gly254 (BTN3A1) basic tail.

Product Background

BTN2A1 and BTN3A1 clearly play highly distinct roles in Vγ9Vδ2 T cell detection of P-Ag-exposed cells. BTN2A1&BTN3A1, a protein complex which contains heterodimeric BTN2A1 and BTN3A1 extracellular domains (ECD), is critical to enhance cytotoxic killing by Vγ9Vδ2 T cells.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human BTN2A1&BTN3A1 complex Protein (BTN-HM123) Specifications/Details

Protein
BTN2A1&BTN3A1
Synonyms
BTN3A1&BTN2A1; BTN2A1&BTN3A1;
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln29-Ala248 (BTN2A1) acidic tail & Gln30-Gly254 (BTN3A1) basic tail
Molecular Weight
The protein has a predicted MW of 30.74 kDa (BTN2A1) and 30.25 kDa (BTN3A1). Due to glycosylation, the protein migrates to 53-63 kDa and 33-38 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human BTN3A1&BTN2A1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His, C-Flag
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human BTN3A1&BTN2A1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). Immobilized Human BTN3A1&BTN2A1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test).
Human BTN2A1&BTN3A1 complex on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human BTN2A1&BTN3A1 complex on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human BTN2A1&BTN3A1 complex is greater than 95% as determined by SEC-HPLC. The purity of Human BTN2A1&BTN3A1 complex is greater than 95% as determined by SEC-HPLC.
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