Product Description |
Recombinant Human BTN3A1/CD277 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln30-Gly254. |
Product Background |
The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. BTN3A1 controls activation of human Vγ9/Vδ2 T cells by direct or indirect presentation of self and nonself phosphoantigens (pAg). |
Product Category |
Recombinant Protein |
Target |
BTN3A1/CD277 |
Synonyms |
CD277; BTN3A1; BTF5; BT3.1; BTN3.1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln30-Gly254 |
Molecular Weight |
The protein has a predicted MW of 25.3 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human BTN3A1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human BTN3A1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA. |
|
|
Human BTN3A1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human BTN3A1 is greater than 95% as determined by SEC-HPLC. |
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