Human BTN3A1/CD277 Protein (BTN-HM1A1)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human BTN3A1 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 8.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln30-Gly254

Catalog: BTN-HM1A1

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Product Description Recombinant Human BTN3A1/CD277 Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Gln30-Gly254.
Product Category Recombinant Protein
Target BTN3A1/CD277
Synonyms CD277; BTN3A1; BTF5; BT3.1; BTN3.1
Gene/Protein ID O00481-1
Species Human
Exact Sequence Gln30-Gly254
Molecular Weight The protein has a predicted MW of 25.3 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human BTN3A1 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 8.1ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human BTN3A1 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 8.1ng/ml determined by ELISA.
The purity of Human BTN3A1 is greater than 95% as determined by SEC-HPLC.
Human BTN3A1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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