Human BTN3A1/CD277 Protein (BTN-HM1A1)
Human BTN3A1/CD277 Protein (BTN-HM1A1)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human BTN3A1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln30-Gly254
Catalog #BTN-HM1A1
Regular price $297.00
/
Size

Order now, ships by:

Human BTN3A1/CD277 Protein (BTN-HM1A1) Summary

Product Description

Recombinant Human BTN3A1/CD277 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln30-Gly254.

Product Background

The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. BTN3A1 controls activation of human Vγ9/Vδ2 T cells by direct or indirect presentation of self and nonself phosphoantigens (pAg).

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Human BTN3A1/CD277 Protein (BTN-HM1A1) Specifications/Details

Protein
BTN3A1/CD277
Synonyms
CD277; BTN3A1; BTF5; BT3.1; BTN3.1
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln30-Gly254
Molecular Weight
The protein has a predicted MW of 25.3 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human BTN3A1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human BTN3A1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA. Immobilized Human BTN3A1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA.
Human BTN3A1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human BTN3A1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human BTN3A1 is greater than 95% as determined by SEC-HPLC. The purity of Human BTN3A1 is greater than 95% as determined by SEC-HPLC.
Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Recently viewed