Human BTN3A1/CD277 Protein (BTN-HM2A3)
Catalog: BTN-HM2A3
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| Product Description | Recombinant Human BTN3A1/CD277 Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Gln30-Gly254. |
| Product Category | Recombinant Protein |
| Target | BTN3A1/CD277 |
| Synonyms | CD277; BTN3A1; BTF5; BT3.1; BTN3.1 |
| Gene/Protein ID | O00481-1 |
| Species | Human |
| Exact Sequence | Gln30-Gly254 |
| Molecular Weight | The protein has a predicted MW of 50.9 kDa. Due to glycosylation, the protein migrates to 58-63 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human BTN3A1, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 20.3ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human BTN3A1, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 20.3ng/ml determined by ELISA. |
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The purity of Human BTN3A1/CD277 is greater than 95% as determined by SEC-HPLC. |
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Human BTN3A1/CD277 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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