Name: Human BTN3A1/CD277 Protein (BTN-HM2A3)
Brand: KactusBio
SKU: BTN-HM2A3-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human BTN3A1, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 22.9ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gln30-Gly254

Catalog #BTN-HM2A3

Size

100UG

500UG

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Product Description	Recombinant Human BTN3A1/CD277 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln30-Gly254.
Product Background	The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. BTN3A1 controls activation of human Vγ9/Vδ2 T cells by direct or indirect presentation of self and nonself phosphoantigens (pAg).
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	BTN3A1/CD277
Synonyms	CD277; BTN3A1; BTF5; BT3.1; BTN3.1
Accession	O00481-1
Species	Human
Biotinylated	no
Amino Acid Range	Gln30-Gly254
Molecular Weight	The protein has a predicted MW of 50.9 kDa. Due to glycosylation, the protein migrates to 58-63 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human BTN3A1, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 22.9ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human BTN3A1, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 22.9ng/ml determined by ELISA.
	Human BTN3A1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human BTN3A1 is greater than 95% as determined by SEC-HPLC.