Human BTN3A1/CD277 Protein (BTN-HM2A3)
Human BTN3A1/CD277 Protein (BTN-HM2A3)
Human BTN3A1/CD277 Protein (BTN-HM2A3)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human BTN3A1, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 22.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln30-Gly254
Catalog #BTN-HM2A3
Regular price $297.00
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Product Description

Recombinant Human BTN3A1/CD277 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln30-Gly254.

Product Background

The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. BTN3A1 controls activation of human Vγ9/Vδ2 T cells by direct or indirect presentation of self and nonself phosphoantigens (pAg).

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

BTN3A1/CD277

Synonyms

CD277; BTN3A1; BTF5; BT3.1; BTN3.1

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Gln30-Gly254

Molecular Weight

The protein has a predicted MW of 50.9 kDa. Due to glycosylation, the protein migrates to 58-63 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human BTN3A1, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 22.9ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human BTN3A1, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 22.9ng/ml determined by ELISA. Immobilized Human BTN3A1, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-BTN3A1 Antibody, hFc Tag with the EC50 of 22.9ng/ml determined by ELISA.
Human BTN3A1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human BTN3A1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human BTN3A1 is greater than 95% as determined by SEC-HPLC. The purity of Human BTN3A1 is greater than 95% as determined by SEC-HPLC.

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