Product Description |
Recombinant Human CA125/MUC16 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp12783-Ser13467. |
Product Background |
MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy.Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.Binding to MSLN mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to MSLN. |
Product Category |
Recombinant Protein |
Target |
CA125/MUC16 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp12783-Ser13467 |
Molecular Weight |
The protein has a predicted MW of 76.17 kDa. Due to glycosylation, the protein migrates to 90-140 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CA125, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human MSLN, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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|
Immobilized Human CA125, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human MSLN, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA. |
|
|
Human CA125 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human CA125 is greater than 95% as determined by SEC-HPLC. |
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