Human CA125/MUC16 Protein (MUC-HM416)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human CA125, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human MSLN, hFc Tag with the EC50 of 1.1ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gly12660-Met12923

Catalog: MUC-HM416

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Product Description	Recombinant Human CA125/MUC16 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly12660-Met12923.
Product Category	Recombinant Protein

Target	CA125/MUC16
Synonyms	CA125; CA-125; CA125MUC-16; FLJ14303; MUC16
Gene/Protein ID	Q8WXI7
Species	Human
Exact Sequence	Gly12660-Met12923
Molecular Weight	The protein has a predicted MW of 31.3 kDa. Due to glycosylation, the protein migrates to 50-80 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CA125, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human MSLN, hFc Tag with the EC50 of 1.1ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CA125, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human MSLN, hFc Tag with the EC50 of 1.1ug/ml determined by ELISA.
	Human CA125 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.