Human CA9/Carbonic Anhydrase IX Protein (CA9-HM401)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CA9, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln38-Asp414

Catalog: CA9-HM401

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Product Description Recombinant Human CA9/Carbonic Anhydrase IX Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln38-Asp414.

Product Background

CA9 is a member of the carbonic anhydrases' family, that is often expressed in cancer cells under hypoxic condition. CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour-associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K/AKR/mTOR signalling and TGF-beta signalling pathways.

Product Category

Recombinant Protein

Target

CA9/Carbonic Anhydrase IX
Synonyms CA9; CAIX; CA-IX; EC 4.2.1.1; G250; MN; P54/58N; PMW1; RCC
Accession

Species

Human

Amino Acid Range

Gln38-Asp414
Molecular Weight The protein has a predicted MW of 43.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human CA9, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 50mM Tris, 150mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CA9, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA.
Measured by its esterase activity. The specific activity is > 20 pmol/min/µg.
The purity of Human CA9 is greater than 95% as determined by SEC-HPLC.
Human CA9 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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