Human CD209/DC-SIGN Protein (SIG-HM101) Summary
Product Description |
Recombinant Human CD209/DC-SIGN Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln59-Ala404. |
Product Background |
C-type lectin CD209/DC-SIGN and CD209L/L-SIGN proteins are distinct cell adhesion and pathogen recognition receptors that mediate cellular interactions and recognize a wide range of pathogens, including viruses such as SARS, SARS-CoV-2, bacteria, fungi and parasites. Pathogens exploit CD209 family proteins to promote infection and evade the immune recognition system. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human CD209/DC-SIGN Protein (SIG-HM101) Specifications/Details
Protein |
CD209/DC-SIGN |
Synonyms |
CD209; CLEC4L; DC-SIGN; DC-SIGN1; CDSIGN; MGC129965; CD209 molecule |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln59-Ala404 |
Molecular Weight |
The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 0.12 uM as determined in SPR assay (Biacore T200). |
Product Tag |
N-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
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Human BTN2A1, His Tag immobilized on CM5 Chip can bind Human CD209, His Tag with an affinity constant of 0.12 uM as determined in SPR assay (Biacore T200). |
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Human CD209 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human CD209 is greater than 95% as determined by SEC-HPLC. |
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