Human CD27 Ligand/CD70 Protein (CD7-HM270)
Human CD27 Ligand/CD70 Protein (CD7-HM270)
Human CD27 Ligand/CD70 Protein (CD7-HM270)
  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human CD27, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human CD27 Ligand, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln39-Pro193
Catalog #CD7-HM270
Regular price $315.00
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Product Description
Recombinant Human CD27 Ligand/CD70 Protein is expressed from HEK293 with hFc at the N-Terminus.It contains Gln39-Pro193.
Product Background
CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7.- CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.
Product Category
Recombinant Protein
Target
CD27 Ligand/CD70
Synonyms
CD70 molecule; CD70; TNFSF7; CD27 Ligand; CD27-L; CD27LG;-Ki-24 antigen; TNFSF7G
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln39-Pro193
Molecular Weight
The protein has a predicted MW of 42.7 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human CD27, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human CD27 Ligand, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA. See testing image for detail.
Product Tag
N-hFc
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD27, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human CD27 Ligand, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA.
Human CD27 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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