Human CD27 Ligand/CD70 Protein (CD7-HM270)
Human CD27 Ligand/CD70 Protein (CD7-HM270)
Human CD27 Ligand/CD70 Protein (CD7-HM270)
  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD27, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human CD27 Ligand, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln39-Pro193
Catalog #CD7-HM270
Regular price $315.00
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Product Description
Recombinant Human CD27 Ligand/CD70 Protein is expressed from HEK293 with hFc at the N-Terminus.It contains Gln39-Pro193.
Product Background
CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7.- CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.
Product Category
Recombinant Protein
Target
CD27 Ligand/CD70
Synonyms
CD70 molecule; CD70; TNFSF7; CD27 Ligand; CD27-L; CD27LG;-Ki-24 antigen; TNFSF7G
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln39-Pro193
Molecular Weight
The protein has a predicted MW of 42.7 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human CD27, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human CD27 Ligand, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA. See testing image for detail.
Product Tag
N-hFc
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD27, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human CD27 Ligand, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA.
Human CD27 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD27 Ligand is greater than 95% as determined by SEC-HPLC.
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