Product Description |
Recombinant Human CD30 Ligand/TNFSF8 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln63-Asp234. |
Product Background |
CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.CD30L is a cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets |
Protein |
CD30 Ligand/TNFSF8 |
Synonyms |
CD153; CD30 Ligand; CD30L; CD30-L; CD30LGMGC138144; TNFSF8; CD30LG |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln63-Asp234 |
Molecular Weight |
The protein has a predicted MW of 20.7 kDa. Due to glycosylation, the protein migrates to 30-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. |
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Human CD30 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human CD30 Ligand is greater than 95% as determined by SEC-HPLC. |
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