Human CD30 Ligand/TNFSF8 Protein (CD3-HM10L)

  • Express System: Expi293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD30 Ligand, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln63-Asp234

Catalog: CD3-HM10L

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Product Description Recombinant Human CD30 Ligand/TNFSF8 Protein is expressed from Expi293 with His tag at the N-terminal.It contains Gln63-Asp234.
Product Category Recombinant Protein
Target CD30 Ligand/TNFSF8
Synonyms CD153; CD30 Ligand; CD30L; CD30-L; CD30LGMGC138144; TNFSF8; CD30LG
Gene/Protein ID P32971
Species Human
Exact Sequence Gln63-Asp234
Molecular Weight The protein has a predicted MW of 20.7 kDa. Due to glycosylation, the protein migrates to 30-50 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human CD30 Ligand, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD30 Ligand, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 12.7ng/ml determined by ELISA.
The purity of Human CD30 Ligand is greater than 95% as determined by SEC-HPLC.
Human CD30 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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