Name: Human CD30 Ligand/TNFSF8 Protein (CD3-HM10L)
Brand: KactusBio
SKU: CD3-HM10L-100UG
Price: 346.5 USD
Availability: InStock

Human CD30 Ligand/TNFSF8 Protein (CD3-HM10L)

Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.

Human CD30 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human CD30 Ligand is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gln63-Asp234

Catalog #CD3-HM10L

Size

100UG

500UG

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Product Description	Recombinant Human CD30 Ligand/TNFSF8 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln63-Asp234.
Product Background	CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.CD30L is a cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Protein	CD30 Ligand/TNFSF8
Synonyms	CD153; CD30 Ligand; CD30L; CD30-L; CD30LGMGC138144; TNFSF8; CD30LG
Accession	P32971
Species	Human
Biotinylated	no
Amino Acid Range	Gln63-Asp234
Molecular Weight	The protein has a predicted MW of 20.7 kDa. Due to glycosylation, the protein migrates to 30-50 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.
	Human CD30 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human CD30 Ligand is greater than 95% as determined by SEC-HPLC.