Human CD30 Ligand/TNFSF8 Protein (CD3-HM10L)
Human CD30 Ligand/TNFSF8 Protein (CD3-HM10L)
Human CD30 Ligand/TNFSF8 Protein (CD3-HM10L)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln63-Asp234
Catalog #CD3-HM10L
Regular price $346.50
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Product Description

Recombinant Human CD30 Ligand/TNFSF8 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln63-Asp234.

Product Background

CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.CD30L is a cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets

Protein

CD30 Ligand/TNFSF8

Synonyms

CD153; CD30 Ligand; CD30L; CD30-L; CD30LGMGC138144; TNFSF8; CD30LG

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Gln63-Asp234

Molecular Weight

The protein has a predicted MW of 20.7 kDa. Due to glycosylation, the protein migrates to 30-50 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.
Human CD30 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD30 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD30 Ligand is greater than 95% as determined by SEC-HPLC. The purity of Human CD30 Ligand is greater than 95% as determined by SEC-HPLC.

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