Human CD30 Ligand/TNFSF8 Protein (CD3-HM30L)

  • Express System: Expi293
  • Product Tag: N-mFc
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC;
  • Activity: Immobilized Human CD30, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD30 Ligand, mFc Tag with the EC50 of 0.12μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln63-Asp234

Catalog: CD3-HM30L

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Product Description Recombinant Human CD30 Ligand/TNFSF8 Protein is expressed from Expi293 with mFc tag at the N-terminal.; It contains Gln63-Asp234.
Product Category Recombinant Protein
Target CD30 Ligand/TNFSF8
Synonyms CD153; CD30 Ligand; CD30L; CD30-L; CD30LGMGC138144; TNFSF8; CD30LG
Gene/Protein ID P32971-1
Species Human
Exact Sequence Gln63-Asp234
Molecular Weight The protein has a predicted MW of 46.2 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human CD30, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD30 Ligand, mFc Tag with the EC50 of 0.12μg/ml determined by ELISA. See testing image for detail.
Product Tag N-mFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC;
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD30, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD30 Ligand, mFc Tag with the EC50 of 0.12μg/ml determined by ELISA.
The purity of Human CD30 Ligand/TNFSF8 is greater than 95% as determined by SEC-HPLC.
Human CD30 Ligand/TNFSF8 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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