Human CD30 Ligand/TNFSF8 Protein (CD3-HM30L)
Human CD30 Ligand/TNFSF8 Protein (CD3-HM30L)
Human CD30 Ligand/TNFSF8 Protein (CD3-HM30L)
Human CD30 Ligand/TNFSF8 Protein (CD3-HM30L)
  • Express System: HEK293
  • Product Tag: N-mFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC;
  • Activity: Immobilized Human CD30, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human CD30 Ligand, mFc Tag with the EC50 of 10.5ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln63-Asp234
Catalog #CD3-HM30L
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Product Description
 Recombinant Human CD30 Ligand/TNFSF8 Protein is expressed from HEK293 with mFc (IgG2a) tag at the N-Terminus.It contains Gln63-Asp234.
Product Background
 CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.CD30L is a cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.
Product Category
 Recombinant Protein
Target
 CD30 Ligand/TNFSF8
Synonyms
 CD153; CD30 Ligand; CD30L; CD30-L; CD30LGMGC138144; TNFSF8; CD30LG
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gln63-Asp234
Molecular Weight
 The protein has a predicted MW of 46.2 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CD30, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human CD30 Ligand, mFc Tag with the EC50 of 10.5ng/ml determined by ELISA. See testing image for detail.
Product Tag
 N-mFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC;
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD30, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human CD30 Ligand, mFc Tag with the EC50 of 10.5ng/ml determined by ELISA.
Human CD30 Ligand on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD30 Ligand is greater than 95% as determined by SEC-HPLC.

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