Human CD30 Ligand/TNFSF8 Protein (CD3-HM30L)

Express System: HEK293
Product Tag: N-mFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC;
Activity: Immobilized Human CD30, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human CD30 Ligand, mFc Tag with the EC50 of 10.5ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gln63-Asp234

Catalog: CD3-HM30L

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Product Description	Recombinant Human CD30 Ligand/TNFSF8 Protein is expressed from HEK293 with mFc (IgG2a) tag at the N-Terminus.It contains Gln63-Asp234.
Product Background	CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.CD30L is a cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.
Product Category	Recombinant Protein

Target	CD30 Ligand/TNFSF8
Synonyms	CD153; CD30 Ligand; CD30L; CD30-L; CD30LGMGC138144; TNFSF8; CD30LG
Gene/Protein ID	P32971-1
Species	Human
Exact Sequence	Gln63-Asp234
Molecular Weight	The protein has a predicted MW of 46.2 kDa. Due to glycosylation, the protein migrates to 62-68 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CD30, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human CD30 Ligand, mFc Tag with the EC50 of 10.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-mFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC;
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CD30, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human CD30 Ligand, mFc Tag with the EC50 of 10.5ng/ml determined by ELISA.
	The purity of Human CD30 Ligand is greater than 95% as determined by SEC-HPLC.
	Human CD30 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.