Human CD30/TNFRSF8 Protein (CD3-HM230)
Human CD30/TNFRSF8 Protein (CD3-HM230)
Human CD30/TNFRSF8 Protein (CD3-HM230)
Human CD30/TNFRSF8 Protein (CD3-HM230)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Phe19-Lys379
Catalog #CD3-HM230
Regular price $203.00
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Product Description
Recombinant Human CD30/TNFRSF8 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe19-Lys379.
Product Background
The transmembrane receptor CD30 (TNFRSF8) and its ligand CD30L (CD153, TNFSF8) are members of the tumor necrosis factor (TNF) superfamily and display restricted expression in subpopulations of activated T-and B-cells in nonpathologic conditions. CD30 expression is upregulated in various hematological malignancies, including Reed-Sternberg cells in Hodgkin's disease (HD), anaplastic large cell lymphoma (ALCL) and subsets of Non-Hodgkin's lymphomas (NHLs).
Product Category
Recombinant Protein
Target
CD30/TNFRSF8
Synonyms
CD30; CD30KI-1; CD30L receptor; TNFRSF8; D1S166EKi-1; CD30KI-1
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Phe19-Lys379
Molecular Weight
The protein has a predicted MW of 65.2 kDa. Due to glycosylation, the protein migrates to 80-115 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-hFc
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD30 Ligand, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.
Human CD30 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD30 is greater than 95% as determined by SEC-HPLC.

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